OCT4 antibody (AA 201-300)

Catalog No. ABIN728773

This Rabbit Polyclonal antibody specifically detects OCT4 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for OCT4 antibody (AA 201-300) (ABIN728773)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-OCT4 Antibody

Binding Specificity: AA 201-300

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Oct-4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: 4-Oct

Background:

Synonyms: MGC22487, Oct 3, Oct 4, Oct-3, Oct-4, OCT3, Oct4, Octamer binding protein 3, Octamer-binding protein 3, Octamer binding protein 4, Octamer binding transcription factor 3, Octamer-binding transcription factor 3, Octamer-4, POU domain transcription factor OCT4, OTF3, OTF4, OTF 3, OTF 4, OTF-3, POU class 5 homeobox 1, POU domain, class 5, transcription factor 1, POU domain class 5 transcription factor 1, PO5F1_HUMAN, POU type homeodomain containing DNA binding protein , POU5F1.

Background: Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

Molecular Weight: 41kDa

Gene ID: 5460

Pathways: Stem Cell Maintenance